Spectrometer (Class 7)

Synopsys

Spectroscopy is an essential tool for monitoring the growth and production in your bioreactor. We will make our own light source and detector setup. The class is about other analytical techniques too.

Schedule

• Analytical techniques
• Spectrometer Design
• Spectrometry Practical
  • Iodine Clock Reaction

Spectrometer Design

This week we will build a spectrometer. We have created 2 spectrometer designs, you may choose which design to build.

• Functional and technical requirements

Photodiode array-based photospectrometer

The photodiode array spectrophotometer files are stored in a git repository which you can clone or download as a zip archive. Check out the Building instructions for info about assembly.

Webcam-based spectrometer design

• SketchUp assembly
• SVG laser cut files

Bill of Materials

In order to take part in this class you will need the following materials:

• CCD Array Spectrometer materials
• Webcam Spectrometer materials

Assignment

Hack the spectrometer into a flow spectrometer.

Additional reading and hacking

• Spectroscopy
  • PublicLab
  • Calibration Assays
• DIY Spectrometers
  • Really Basic - Instructable
  • Spectruino
  • CCD array
  • TSL1402R
• DIY absorbance meters
  • Open Source Colorimeter

Feeling lazy?

Go ahead and buy a spectrometer:

• Second hand photospectrometers at LabX