Hello everyone,

Fully packed incubators and surrounded by project boxes I am writing you this week’s update. We have an exciting week ahead of us, making our first steps towards isolating biomaterials using centrifugation and chromatography.

Schedule for next Monday and Tuesday

Monday:

10:00-10:30 Doors open 10:30-11:30 DNA extraction 11:30-11:45 Coffee break 11:45-12:45 Chloroplast isolation 12:45-13:45 Lunch break 13:45-14:45 Chromatography 13:45-18:00 Open lab, time to work on your projects and assignments

Tuesday:

10:00-10:30 Doors open 10:30-12:30 Centrifuge construction and help with electronics 12:30-13:30 Lunch break 13:30-14:30 Assignment meeting 14:30-18:00 Open Lab 19:00-21:00 Class 6: Magnetic Stirrer

Assigment

This week you have only one assignment:

1) Start sketching your bioreactor controller in Fritzing.

The challenge for you is to submerge yourself into Fritzing. A bioreactor controller typically consists of the following parts: Temperature sensor, Heater, pH sensor, Stirrer, Substrate pump (for Fed Batch or Chemostat), Acid or Base pump, Optical sensor and Light sensor / switch.

Spectrometers

We made two designs for the spectrometer. One based on a webcam hack, that is relatively easy, and a second based on a CCD Array that’s linked to an Arduino. The former is relatively easy, while the latter is more suitable for hard core Arduino freaks.

http://biohackacademy.github.io/biofactory/class/7-spectrometer/

You may now order the components as listed in the Bill of Materials.

Slides & videos

As usual the video of yesterday’s lecture can be found on Vimeo. https://vimeo.com/channels/biohackacademy http://biohackacademy.github.io/biofactory/class/5-centrifuge/

See you next week,

The BHA Team